Gestational age estimation in human fetuses from histogenesis of the spleen

  • Aksh Dubey Swami Rama Himalayan University
  • Sunder Lal Jethani Swami Rama Himalayan University
  • Deepa Singh Swami Rama Himalayan University


Background: Stages in development and maturation of various organs and tissues may help in fetal

gestational age estimation in medicolegal cases. The present study was undertaken to study the

maturation of fetal splenic tissue

Methods: 30 human fetuses of gestational age ranging from 9th to 38th weeks were used for the

study. Gestational age estimation was done by the crown rump length followed by distribution of

fetuses into five groups. Spleen was dissected out from each fetus followed by tissue processing.

Sections of 4-5µm were cut and stained with haematoxylin and eosin stain. All slides were

observed under low and high powers of the light microscope

Results: The red pulp was the first to develop, appearing at the 9th week, followed by the

capsule. Lobulation and trabeculae appeared during the period of 15 to 17 weeks. Lastly white

pulp developed at the 25th week.

Conclusion: The time of appearance of the various features in the developing spleen may help in

rough estimation of the gestational age from minimal fetal splenic tissue remains.


Oct 16, 2018
How to Cite
DUBEY, Aksh; JETHANI, Sunder Lal; SINGH, Deepa. Gestational age estimation in human fetuses from histogenesis of the spleen. SRHU MEDICAL JOURNAL, [S.l.], v. 1, n. 2, p. 76 - 79, oct. 2018. ISSN 2456-7825. Available at: <>. Date accessed: 19 jan. 2019.
Original Research